Effect of adding titanium dioxide nanoparticles on anti-microbial activity and surface detail reproduction of dental alginate

Main Article Content

Ranj A. Omer
https://orcid.org/0009-0004-8106-7887
Hoshang Kh. Abdel-Rahman
https://orcid.org/0000-0001-9025-3754
Mahabad M. Saleh
https://orcid.org/0000-0002-9913-6084
Sazgar S.Q. Al-Hawezi
Fahd S. Ikram
https://orcid.org/0009-0005-1952-1003

Abstract

Most dental works require a diagnostic impression; alginate is contemplated as the most popular material used for this purpose. Titanium dioxide nanoparticles show evidence of antimicrobial activity in the recent era, for this purpose, this study aimed to evaluate the effect of adding Titanium dioxide nanoparticles on antimicrobial activity and surface detail reproduction of alginate impression material. Materials and methods: Titanium dioxide nanoparticles (purity = 99%, size= 20nm) was added to alginate at three different concentrations (2%, 3% and 5%). 84 samples were prepared in total. Samples were tested for antimicrobial activity using a disc diffusion test, and surface detail reproduction was done using (ISO 21563:2021). One-way ANOVA and independent sample t-test were used for data analysis through SPSS software. Results: for the antimicrobial test, inhibition zones for Streptococcus mutans and Candida albicans showed significant changes concerning the alteration in Titanium dioxide nanoparticle concentrations. The inhibition zone significantly increased with an increase in the percentage of Titanium dioxide nanoparticles. The mean of the inhibition zone for S. mutans was superior to C. albicans and the difference was statistically significant. Regarding surface detail reproduction, the control group, 2% and 3% groups manifested very similar results, only the group to which 5% of Titanium dioxide nanoparticles were added showed a decline in detail reproduction when compared to the other three groups. Conclusion: Within the limitation of this study, we can conclude that the antimicrobial activity against S mutans and C. albicans were significantly increased in modified groups, and this escalation was directly linked to the increase in Titanium dioxide nanoparticles concentration. In contrast, the surface detail reproduction was decreased when adding 5% Titanium dioxide nanoparticles to alginate.

Downloads

Download data is not yet available.

Article Details

Section

Research Articles

Author Biographies

Ranj A. Omer , Gasha Technical Institute, Department of Dental Technology, Iraq

Gasha Technical Institute, Department of Dental Technology, Iraq

Hoshang Kh. Abdel-Rahman, Department of Prosthodontics, College of Dentistry, Hawler Medical University, Iraq

Department of Prosthodontics, College of Dentistry, Hawler Medical University, Iraq

Mahabad M. Saleh, Department of Prosthodontics, College of Dentistry, Hawler Medical University, Iraq

Department of Prosthodontics, College of Dentistry, Hawler Medical University, Iraq

Sazgar S.Q. Al-Hawezi, Department of Conservative Dentistry, College of Dentistry, Hawler Medical University, Iraq.

Department of Conservative Dentistry, College of Dentistry, Hawler Medical University, Iraq.

Fahd S. Ikram, Department of Prosthodontics, College of Dentistry, Hawler Medical University, Iraq

Department of Prosthodontics, College of Dentistry, Hawler Medical University, Iraq

How to Cite

1.
Omer RA, Abdel-Rahman HK, Saleh MM, Al-Hawezi SS, Ikram FS. Effect of adding titanium dioxide nanoparticles on anti-microbial activity and surface detail reproduction of dental alginate. J Bagh Coll Dent [Internet]. 2023 Mar. 15 [cited 2024 Dec. 23];35(1):36-48. Available from: https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/3313

References

Hamalian, T. A., Nasr, E., Chidiac, J.J. Impression Materials in Fixed prosthodontics: influence of choice on clinical proce-dure. J Prosthodont. 2011; 20(2):153-60.

Hansson, O., Eklund, J. A historical review of hydrocolloids and an investigation of the dimensional accuracy of the new alginates for crown and bridge impressions when using stock trays. Swed Dent J. 1984; 8(2): 81-95.

Cervino, G., Fiorillo, L., Herford, A., et al. Alginate Materials and Dental Impression Technique: A Current State of the Art and Application to Dental Practice. Mar Drugs. 2018; 17(1): 18.

Glenner, R.A. Dental impressions. J Hist Dent. 1997; 45(3): 127–30.

Samra, R.K. Bhide, S.V. Efficacy of Different Disinfectant Systems on Alginate and Addition Silicone Impression Materials of Indian and International Origin: A Comparative Evaluation. J Indian Prosthodont Soc. 2010; 10(3): 182–189.

Amalan , K., Ginjupalli, A. Upadhya, N. Evaluation of properties of irreversible hydrocolloid impression materials mixed with disinfectant liquids. Dent Res J (Isfahan). 2013; 10(1): 65-73.

Jenning, K.J. Samaranayake, L.P. The persistence of microorganisms on impression materials following disinfection. Int J Prosthodont. 1991; 4(4): 382-7.

Wang, J., Wan, Q., Chao, Y., et al. A self-disinfecting irreversible hydrocolloid impression material mixed with chlorhexidine solution. Angle Orthod. 2007; 77(5): 894-900.

Mitra, S.B. Nanoparticles for dental materials: synthesis, analysis, and applications. In: Emerging nanotechnologies in den-tistry. Elsevier; 2012. p. 15–33.

Figueroa,, L. A., Morales-Luckie., R. A., Scougall-Vilchis, R.J., et al. Synthesis, characterization and antibacterial activity of copper, nickel and bimetallic Cu–Ni nanoparticles for potential use in dental materials. Progress in Natural Science: Materials International.20114; 24(4): 321-328.

Khan I, Saeed K, Khan I.. Nanoparticles: Properties, applications and toxicities. Arab J Chem. 2019; 12(7): 908-931.

Jefferson, K.K. What drives bacteria to produce a biofilm?. FEMS Microbiology Letters. 2004; 236(2): 163–173.

Al-Hawezi, S.S.Q. The effect of addition of titanium dioxide nanofillers on the properties of flowable composite resin (in vitro study). Ph.D.Thesis. Hawler Medical University, Iraq, 2021.

Sodagar, A., Akhoundi, M.S.A., Bahador, A, et al. Effect of TiO2 nanoparticles incorporation on antibacterial properties and shear bond strength of dental composite used in Orthodontics. Dent Press J Orthod. 2017; 22(5): 67–74.

Ahmed, A.Q. The influence of titanium dioxide nanoparticles (TiO2NPS) incorporation into heat cured soft denture lining material on Candida albicans adherence and some other properties. M.Sc thesis. University of Baghdad, Iraq, 2018..

Whitfield, P., Mitchell, L. X-ray diffraction analysis of nanoparticles: Recent developments, potential problems and some solutions. Int J Nanosci. 2004; 3(6): 757–63.

Bunaciu, A.A., UdriŞTioiu, E.G. Aboul-Enein, H.Y. X-ray diffraction: instrumentation and applications. Crit Rev Anal Chem 2015; 45(4): 289–99.

Ruparelia, J.P., Chatterjee, A.K., Duttagupta, S.P., et al. Strain specificity in antimicrobial activity of silver and copper na-noparticles. Acta Biomater. 2008; 4(3): 707–16

Skocaj, M., Filipic, M., Petkovic, J., et al. Titanium dioxide in our everyday life; is it safe?. Radiol Oncol. 2011; 45(4): 227-247.

Abdelrahman, H.K. Al-Sammaraie, S.A.S. Effect of addition of Magnesium Oxide Nanoparticles on surface hardness and tensile bond strength of denture soft liner. IJFMT. 2020; 14(3): 2479-2485.

Monteiro, D.R., Gorup, L.F., Takamiya, A.S., et al. Silver distribution and release from an antimicrobial denture base resin containing silver colloidal nanoparticles. J Prosthodont. 2012; 21(1): 7–15.

Pfaller, M.A. National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal sus-ceptibility testing of yeasts: approved standard. Wayne, Pa.: National Committee for Clinical Laboratory Standards; 2002.

Coyle, M.B. American Society for Microbiology. Manual of antimicrobial susceptibility testing. Burnaby, BC: BCIT Imaging Services; 2012.

Chneider, C.A., Rasband, W.S., Eliceiri, K.W. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012; 9(7): 671–5.

Bykowski, T. Stevenson, B. Aseptic technique. Current Protocols Microbiol. 2008; 11(1): A-4D.

Omer, R.A. Ikram, F.S. Evaluation of Antibacterial Effect of Silver and Copper Oxide Nanoparticles in Denture Base Material Against Streptococcus mutans and Escherichia coli. Saudi Dental J. 2019; 6(1):13–20.

ISO 21563: 2021. Dentistry-Hydrocolloid Impression Material. Geneva, Switzerland: International Organization for Stand-ardization.

American Dental Association Specification No. 19 .Elastomeric Impression Materials. 2004s

Owen, C.P. An investigation into the compatibility of some irreversible hydrocolloid impression materials and dental gyp-sum products: Part II. A refined discriminatory procedure. J Oral Rehabil. 1986; 13(2):147–62.

ISO 4823: 2021. Dentistry-Elastomeric Impression Material. Geneva, Switzerland: International Organization for Standard-ization.

Gupta, S., Rani, S., Garg, S. Infection control knowledge and practice: a cross-sectional survey on dental laboratories in dental institutes of North India. J Indian Prosthodont Soc 2017; 17: 348- 354.

Courrol, D.S., Lopes, C.R.B., Cordeiro, T.S., et al. Optical properties and antimicrobial effects of silver nanoparticles synthe-sized by femtosecond laser photoreduction. Optics Laser Technol. 2018; 103: 233–8.

de Castro DT, Kreve S, Oliveira VC, et al. Development of an impression material with antimicrobial properties for dental application. J Prosthodont. 2019 Oct;28(8):906-12.

Rajendran, V., Suma, K., Ali, S.A., et al. Antimicrobial Efficacy of Irreversible Hydrocolloid Impression Impregnated with Silver Nanoparticles Compared to Surface Disinfected Impressions An In vivo Study.

J Pharm Bioallied Sci. 2021; 13(1): S532-6.

Hu, H., Fan, X., Yin, Y., et al. Mechanisms of titanium dioxide nanoparticle-induced oxidative stress and modulation of plasma glucose in mice. Environ Toxicol. 2019, 34(11): 1221-1235.

Seil, J.T., Webster, T.J. Antimicrobial applications of nanotechnology: methods and literature. Int J Nanomed, 2012; 7: 2767-2781

Sondi, I., Salopek-Sondi, B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. JCIS Open. 2004; 275(1):177–82.

Abbaszadegan, A., Ghahramani, Y., Gholami, A., et al. The effect of charge at the surface of silver nanoparticles on antimi-crobial activity against gram-positive and gram-negative bacteria: a preliminary study. J Nanomater. 2015; 16(1): 53.

Ahmad, N.S., Abdullah, N., Yasin, F.M. Antifungal activity of titanium dioxide nanoparticles against Candida albicans. Bio Res. 2019; 14(4): 8866-8878.

Kermani, S.A., Salari, S., Almani, P.G.N. Comparison of antifungal and cytotoxicity activities of titanium dioxide and zinc oxide nanoparticles with amphotericin B against different Candida species: In vitro evaluation. J Clin Lab Anal. 2021; 35: e23577.

Haghighi, F., Mohammadi, R.S., Mohammadi, P., et al. Antifungal activity of TiO2 nanoparticles and EDTA on Candida albicans biofilms. Infection, Epidemiology and Microbiology. 2013; 1(1): 33-8

Kamikawa, Y., Hirabayashi, D., Nagayama, T., et al. In vitro antifungal activity against oral Candida species using a denture base coated with silver nanoparticles. J Nanomater. 2014; 2014: 48.

Manar, J. Alginate as impression material. J Appl Oral Sci. 2018; 4(3): 300-3.

Omidkhoda, M., Hasanzadeh, N., Soleimani, F. Antimicrobial and physical properties of alginate impression material in-corporated with silver nanoparticles. Dent Res J. 2019; 16(6): 372-376.

Watson S, Beydoun D, Scott J, et al. Preparation of nanosized crystalline TiO 2 particles at low temperature for photocatal-ysis. J. Nanopart Res. 2004 Jun;6:193-207.

American Dental Association Specification No. 18. dental alginate impression materials. 1992.

Similar Articles

You may also start an advanced similarity search for this article.