The validity of salivary microRNAs (hsa-miR-200a, hsa-miR-125a and hsa- miR-93) as oral squamous cell carcinoma biomarker

PDF

Shaimaa H Mudhir

Raja H Al-Jubouri

Ban A Abdul Majeed

Abstract

Background: Oral squamous cell carcinoma represents the vast majority of oral cancer it is a common malignant tumor with an increasing incidence. Around the world, the 5 year mortality rate of oral cancer is about 50%. Thus novel biomarkers for early detection oral squamous cell carcinoma are needed. The level of three salivary microRNAs namely hsa-miR-200a, hsa-miR-125a and hsa- miR-93 were measured in saliva of patients with oral squamous cell carcinoma and compared their levels in saliva of healthy control subjects to determine their potential as oral cancer biomarker.
Materials and methods: The level of these three microRNAs was measured by using revers transcription, preamplification and quantitative PCR.
Results: Only miR-200a present in a significantly lower level (p<0.05) in the saliva of oral squamous cell carcinoma patients than in control. miR-200a was the strongest parameter (most affected by disease status) in the context of differentiation between OSSC and healthy controls (having the highest ROC area of 0.781 which is significantly higher than the area associated with equivocal test). Coming next in order of importance in the context of case-control differentiation was normalized CT values for hsa-miR-93, which has a reasonably high ROC (0.650), but failed to show statically significance differences, P>0.05.
Conclusions: The detection of miRNAs in saliva can be used as noninvasive and rapid diagnostic tool for the diagnosis of oral cancer.

Downloads

Download data is not yet available.

How to Cite

Mudhir SH, Al-Jubouri RH, Abdul Majeed BA. The validity of salivary microRNAs (hsa-miR-200a, hsa-miR-125a and hsa- miR-93) as oral squamous cell carcinoma biomarker. J Bagh Coll Dent [Internet]. 2014 Sep. 15 [cited 2024 Nov. 19];26(3):66-71. Available from: https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/519

Issue

Vol. 26 No. 3 (2014)

Section

Oral Diagnosis

Licenses and Copyright

The following policy applies in The Journal of Baghdad College of Dentistry (JBCD):

# JBCD applies the Creative Commons Attribution (CC BY) license to articles and other works we publish. If you submit your paper for publication by JBCD, you agree to have the CC BY license applied to your work. Under this Open Access license, you as the author agree that anyone can reuse your article in whole or part for any purpose, for free, even for commercial purposes. Anyone may copy, distribute, or reuse the content as long as the author and original source are properly cited. This facilitates freedom in re-use and also ensures that JBCD content can be mined without barriers for the needs of research.

# If your manuscript contains content such as photos, images, figures, tables, audio files, videos, etc., that you or your co-authors do not own, we will require you to provide us with proof that the owner of that content (a) has given you written permission to use it, and (b) has approved of the CC BY license being applied to their content. We provide a form you can use to ask for and obtain permission from the owner. If you do not have owner permission, we will ask you to remove that content and/or replace it with other content that you own or have such permission to use.Don't assume that you can use any content you find on the Internet, or that the content is fair game just because it isn't clear who the owner is or what license applies.

# Many authors assume that if they previously published a paper through another publisher, they own the rights to that content and they can freely use that content in their paper, but thatâ€™s not necessarily the case, it depends on the license that covers the other paper. Some publishers allow free and unrestricted re-use of article content they own, such as under the CC BY license. Other publishers use licenses that allow re-use only if the same license is applied by the person or publisher re-using the content. If the paper was published under a CC BY license or another license that allows free and unrestricted use, you may use the content in your JBCD paper provided that you give proper attribution, as explained above.If the content was published under a more restrictive license, you must ascertain what rights you have under that license. At a minimum, review the license to make sure you can use the content. Contact that JBCD if you have any questions about the license. If the license does not permit you to use the content in a paper that will be covered by an unrestricted license, you must obtain written permission from the publisher to use the content in your JBCD paper. Please do not include any content in your JBCD paper which you do not have rights to use, and always give proper attribution.

# If any relevant accompanying data is submitted to repositories with stated licensing policies, the policies should not be more restrictive than CC BY.

# JBCD reserves the right to remove any photos, captures, images, figures, tables, illustrations, audio and video files, and the like, from any paper, whether before or after publication, if we have reason to believe that the content was included in your paper without permission from the owner of the content.