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Materials and Methods: Extracted human teeth were biomechanically prepared, vertically sectioned, placed in the tissue culture wells exposing the root canal surface to E. faecalis to form a biofilm. At the end of the 3rd and 6th weeks, all groups were treated for 10 minutes with the previously mentioned tested solutions and control and were analyzed qualitatively and quantitatively.
Results: Qualitative assay with 3-weeks biofilm showed a complete inhibition of bacterial growth with co+steralium and NaOCl, except steralium and saline, which showed presence of bacterial growth. In quantitative analysis, steralium and saline- treated tooth samples have 57.74 Â± 5.39* CFU/ml., 140.71 Â± 8.49 CFU/ml. respectively. Qualitative assay with 6-week biofilm showed growth when treated with steralium and saline treated tooth samples have 346.0 Â± 78.88* CFU/ml., 127.18 Â± 17.84 CFU/ml. respectively whereas co+steralium and NaOCl has shown complete inhibition.
Conclusions: co+steralium and 5% sodium hypochlorite showed maximum antibacterial activity against E. Faecalis biofilm formed on tooth substrate. co+steralium showed statistically significant antibacterial activity. The use of co+steralium as a root canal irrigant might prove to be advantageous considering the several undesirable characteristics of NaOCl.
Key words: Biofilm, E. faecalis, steralium, co+steralium, NaOCl, root canal irrigant.
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