Determination of the effect of stress on the salivary cortisol level among sample of university students having myofacial pain
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Abstract
Materials and Methods: Ninety eight (98) university students were enrolled in this study. Fifty(50) were with myofacial pain (symptomatic) and forty eight (48) were without myofacial pain (asymptomatic) as a control group.Each student with myofacial pain was examined according to Research Diagnostic Criteria for Temporomandibular Disorders. Saliva sample were collected from each subject before final examination and three months later for biochemical analysis of cortisolusing ELISA test.
Results: A highly significant difference in salivary cortisol level between the two periods for both the study and control groups, and a non-significant difference between the two groups in before examination period. A negative association has been observed between the level of salivary cortisol and severity of pain and a highly significant improvement of pain between the final examination periods and three months later.
Conclusions: Dental students perceived a higher level of stress prior to the final exam was associated with raised salivary cortisol levels which could be considered as a useful non-invasive biomarker for measuring acute stress.
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