Diagnostic biomarkers for periodontitis (observational case-control study)
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Abstract
Background: Early detection of periodontal tissue loss prevents further development and halts additional damage. The purpose of this study was to investigate the diagnostic ability of salivary Pyridinoline cross-linked carboxy-terminal telopeptide of type I collagen (ICTP) and deoxypyridinoline (DPD) in differentiating periodontitis from periodontal health. Material and method: A case-control included 80 participants who were divided into two groups: 40 periodontitis patients and 40 subjects with a healthy periodontium. Salivary samples were collected from each patient, followed by a clinical examination. The collected saliva samples were centrifuged and frozen at -80⁰C until analysis using Enzyme-Linked Immunosorbent Assay. Results: the results indicated that both biomarkers were effective in diagnosis periodontitis. The area under the curve (AUC) for ICTP was 0.99 and the proposed cut-off point was 6.6 ng/ml, while for DPD the AUC was 0.95 and the proposed cut-off point was 211.5 nmol/L. Conclusion: Salivary ICTP and DPD demonstrated diagnostic ability in distinguishing periodontitis from a healthy periodontium, making them valuable tools in early detection and management of periodontal diseases.
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References
Tonetti MS, Greenwell H, Kornman KS. Staging and grading of periodontitis: Framework and proposal of a new classification and case definition. J Periodontol 2018; 89 Suppl 1:S159-S172.
Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, et al. Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol 2018; 89 Suppl 1:S173-S182.
Könönen E, Gursoy M, Gursoy UK. Periodontitis: A Multifaceted Disease of Tooth-Supporting Tissues. J Clin Med 2019; 8(8): 1135.
He W, You M, Wan W, Xu F, Li F, Li A. Point-of-Care Periodontitis Testing: Biomarkers, Current Technologies, and Perspectives. Trends Biotechnol 2018; 36(11):1127-1144.
Risteli J, Elomaa I, Niemi S, Novamo A, Risteli L. Radioimmunoassay for the pyridinoline cross-linked carboxy-terminal telopeptide of type I collagen: a new serum marker of bone collagen degradation. Clin Chem 1993; 39(4):635-40.
Jorge G, Sonia R. Pyridinoline (ICTP) Levels in Gingival Crevicular Fluid (GCF) in Chronic Periodontitis. Odovtos - Int J Dent Sc 2016; 18(3): 61-68.
Suchetha A, Aparna Suraj N, Apoorva SM, Bhat D, Sapna N, Darshan BM, BONE TURNOVER BIOMARKERS OF ORAL FLUIDS, World J. Pharm. Res 2020; 9(13): 491-509.
Zhang L, Henson BS, Camargo PM, Wong DT. The clinical value of salivary biomarkers for periodontal disease. Periodontol 2000 2009; 51:25-37.
Chapple ILC, Mealey BL, Van Dyke TE, Bartold PM, Dommisch H, Eickholz P, et al. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol 2018; 89 Suppl 1:S74-S84.
Sharma SK, Mudgal SK, Thakur K, Gaur R. How to calculate sample size for observational and experimental nursing research studies. Natl J Physiol Pharm Pharmacol 2020; 10(1): 1-8.
O'Leary TJ, Drake RB, Naylor JE. The plaque control record. J Periodontol 1972; 43(1):38.
Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J 1975; 25(4):229-35.
Savage A, Eaton KA, Moles DR, Needleman I. A systematic review of definitions of periodontitis and methods that have been used to identify this disease. J Clin Periodontol 2009; 36(6):458-67.
Newman MG, Takei H, Klokkevold PR, Carranza FA. Carranza's clinical periodontology. 11th ed. St. Louis, MO : Elsevier; 2011.
Genco RJ, Borgnakke WS. Risk factors for periodontal disease. Periodontol 2000 2013; 62(1):59-94.
Lertpimonchai A, Rattanasiri S, Arj-Ong Vallibhakara S, Attia J, Thakkinstian A. The association between oral hygiene and periodontitis: a systematic review and meta-analysis. Int Dent J 2017; 67(6):332-343.
Fadhil R, Akram HM, Najah A, Gul SS. Association of matrix metalloproteinase-1-1607 1G/2G single nucleotide polymorphism genotypes with periodontitis in Iraqi population. Braz Dent Sci 2022; 25(3):1-8.
Lekaa M. Ibraheem, Ban Z. Ahmmad, Ayat M. Dhafer, Jannat M. Dhafer. Effect of diabetes mellitus on periodontal health status, salivary flow rate and salivary pH in patients with chronic periodontitis. J Bagh College Dent 2020; 32(2):12-16.
Suha A. Dahash, Maha Sh. Mahmood. Association of a genetic variant (rs689466) of Cyclooxygenase-2 gene with chronic periodontitis in a sample of the Iraqi population. J Bagh College Dent 2019; 31(4):40-45.
Zhu H, Lin X, Zheng P, Chen H. Inflammatory cytokine levels in patients with periodontitis and/or coronary heart disease. Int J Clin Exp Pathol 2015; 8(2):2214-20.
Pacifici R. Estrogen, cytokines, and pathogenesis of postmenopausal osteoporosis. J Bone Miner Res 1996; 11(8):1043-51.
Pacifici R, Brown C, Puscheck E, Friedrich E, Slatopolsky E, Maggio D, McCracken R, Avioli LV. Effect of surgical menopause and estrogen replacement on cytokine release from human blood mononuclear cells. Proc Natl Acad Sci USA 1991; 88(12):5134-8.
Zhang F, Liu E, Radaic A, Yu X, Yang S, Yu C, et al. Diagnostic potential and future directions of matrix metalloproteinases as biomarkers in gingival crevicular fluid of oral and systemic diseases. Int J Biol Macromol 2021; 188:180-196.
Juluri R, Prashanth E, Gopalakrishnan D, Kathariya R, Devanoorkar A, Viswanathan V, Romanos GE. Association of Postmenopausal Osteoporosis and Periodontal Disease: A Double-Blind Case-Control Study. J Int Oral Health. 2015; 7(9):119-23.
Gondim V, Aun J, Fukuda CT, Takayama L, Latorre Mdo R, Pannuti CM, et al. Severe loss of clinical attachment level: an independent association with low hip bone mineral density in postmenopausal females. J Periodontol 2013; 84(3):352-9.
Mahmood AA, Abbas RF. Assessment of NLRP3 Gene Polymorphisms with Periodontitis as Compared with Healthy Periodontium in Iraqi Arabs Patients. Eur J Dent 2023; 17(4):1338-1348.
Charles P, Mosekilde L, Risteli L, Risteli J, Eriksen EF. Assessment of bone remodeling using biochemical indicators of type I collagen synthesis and degradation: relation to calcium kinetics. Bone Miner 1994; 24(2):81-94.
Taba MJ, Kinney J, Kim AS, Giannobile WV. Diagnostic biomarkers for oral and periodontal diseases. Dent Clin North Am 2005; 49(3):551-71.
Garnero P, Ferreras M, Karsdal MA, Nicamhlaoibh R, Risteli J, Borel O, et al. The type I collagen fragments ICTP and CTX reveal distinct enzymatic pathways of bone collagen degradation. J Bone Miner Res 2003; 18(5):859-67.
Eriksen EF, Charles P, Melsen F, Mosekilde L, Risteli L, Risteli J. Serum markers of type I collagen formation and degradation in metabolic bone disease: correlation with bone histomorphometry. J Bone Miner Res 1993; 8(2):127-32.
Mishra D, Gopalakrishnan S, Arun KV, Kumar TS, Devanathan S, Misra SR. Evaluation of Salivary Levels of Pyridinoline Cross Linked Carboxyterminal Telopeptide of Type I Collagen (ICTP) in Periodontal Health and Disease. J Clin Diagn Res 2015; 9(9):ZC50-5.
Chapurlat RD, Genant HK, Osteoporosis, Seventh Ed., vol. 1-2. Elsevier Inc., 2015.
Burr DB. Bone Morphology and Organization. In: Burr DB, Allen MR. Basic and Applied Bone Biology. London: Elsvier; 2019. P. 3-26.
Syed S, Kankara VR, Pathakota KR, Krishnan P, Mishra A. Evaluation of deoxypyridinoline levels in gingival crevicular fluid and serum as alveolar bone loss biomarker in patients with periodontitis. J Indian Soc Periodontol 2020; 24(4):322-328.