Immunohistochemical evaluation for integrin binding sialoprotein on healing process of intrabony defect treated by bone sialoprotein
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Abstract
Background: Bone defect healing is a multidimensional procedure with an overlapping timeline that involves the regeneration of bone tissue. Due to bone's ability to regenerate, the vast majority of bone abnormalities can be restored intuitively under the right physiological conditions. The goal of this study is to examine the immunohistochemistry of bone sialoprotein in order to determine the effect of local application of bone sialoprotein on the healing of a rat tibia generated bone defect. Materials and Methods: In this experiment, 48 albino male rats weighing 300-400 grams and aged 6-8 months will be employed under controlled temperature, drinking, and food consumption settings. The animals will be subjected to a surgical procedure on the medial side of the tibiae bone, with the bone defect repaired with absorbable hemostatic material in the control group (12 rats). The experimental group (12 rats) will be treated with local administration of 30 μl bone sialoprotein fixed by absorbable hemostatic sponge. After surgery, the rats will be slaughtered at 7, 14, and 28 days (four rats for each period). Results: Immunohistochemical analysis of bone sialoprotein by stromal cells reveal a substantial difference between the bone sialoprotein group and the control group. Conclusion: The study concludes that local application of bone sialoprotein could be a successful therapeutic treatment for bone injuries; these findings are encouraging for future clinical use.
Received date: 02-02-2022
Accepted date: 01-03-2022
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Prodinger P M, Bürklein D, Foehr P, Kreutzer K, Pilge H, Schmitt A, ... & Tischer T. Improving results in rat fracture models: enhancing the efficacy of biomechanical testing by a modification of the experimental setup. Bmc musculoskeletal disorders, 2018; 19(1): 243. DOI: https://doi.org/10.1186/s12891-018-2155-y
Oury F, Khrimian L, Denny C A, Gardin A, Chamouni A, Goeden N, ... & Suyama S. Maternal and offspring pools of osteocalcin influence brain development and functions. Cell, 2013; 155(1): 228-241. DOI: https://doi.org/10.1016/j.cell.2013.08.042
Nikel O, Poundarik A A, Bailey S, & Vashishth D. Structural role of osteocalcin and osteopontin in energy dissipation in bone. Journal of biomechanics. 2018; 80: 45-52. DOI: https://doi.org/10.1016/j.jbiomech.2018.08.014
Aubin JE. Osteogenic cell differentiation. In: Davies JE. Bone engineering.2000; 19–30.
Haley S, Gulliver K, Baldassarre R,Miller S, Lane R and Moyer-Mileur L.Tactile and Kinesthetic Stimulation (TKS) intervention improves outcomes in weanling rat bone in a neonatal stress model.J Musculoskelet Neuronal Interact. 2013; 13(2):157-165.
Sodek J and Mckee M. Molecular and cellular biology of alveolar bone.Peridontol. 2000; 24:99-126. DOI: https://doi.org/10.1034/j.1600-0757.2000.2240106.x
Jamil B. Role of biomaterial collagen coated titanium implant surface on expression of bone protein markers and osseointegration reaction, in comparison to titanium implant coated with zirconia. PhD thesis, collage of Dentistry, University of Baghdad .2011.
Gorski J P, Wang A, Lovitch D, Law D, Powell K & Midura R J J J O B C. Extracellular bone acidic glycoprotein-75 defines condensed mesenchyme regions to be mineralized and localizes with bone sialoprotein during intramembranous bone formation. 2004; 279: 25455-25463. DOI: https://doi.org/10.1074/jbc.M312408200
Bouet G, Bouleftour W, Juignet L, Linossier M T, Thomas M , Vanden-Bossche A, Aubin J E, Vico L, Marchat D & Malaval L J P O. The impairment of osteogenesis in bone sialoprotein (BSP) knockout calvaria cell cultures is cell density dependent. 2015; 10: e0117402. DOI: https://doi.org/10.1371/journal.pone.0117402
Gordon J A, Ttye C E, Ssampaio A V, Underhill T M, Hunter G K & Goldberg H A J B. Bone sialoprotein expression enhances osteoblast differentiation and matrix mineralization in vitro. 2007; 41: 462-473. DOI: https://doi.org/10.1016/j.bone.2007.04.191
Baht G S, Hunter G K & Goldberg H A JM B. Bone sialoprotein–collagen interaction promotes hydroxyapatite nucleation. 2008; 27: 600-608. DOI: https://doi.org/10.1016/j.matbio.2008.06.004
Fisher L W & Fedarko N S J C T R. Six genes expressed in bones and teeth encode the current members of the SIBLING family of proteins. 2003; 44: 33-40. DOI: https://doi.org/10.1080/713713644
O'loughlin P F, Morr S, Bogunovic L, Kim A D, Park B & Lane J M. Selection and development of preclinical models in fracture-healing research. 2008; JBJS, 90(Supplement_1): 79-84. DOI: https://doi.org/10.2106/JBJS.G.01585
Zhu B, Guo Z, Lin L & Liu Q J E R M P S. Serum BSP, PSADT, and Spondin-2 levels in prostate cancer and the diagnostic significance of their ROC curves in bone metastasis. 2017; 21: 61-67.
Bäuerle T, Peterschmitt J, Hilbig H , Kiessling F, Armbruster F P & Berger M R J I J O O. Treatment of bone metastasis induced by MDA-MB-231 breast cancer cells with an antibody against bone sialoprotein. 2006; 28: 573-583. DOI: https://doi.org/10.3892/ijo.28.3.573
Zepp M, Kovacheva M, Altankhuyag M,Westphal G, Berger I, Gather K S, Hilbig H, Neuhaus J, Hänsch G M & Armbruster F P J T J O P C R. IDK1 is a rat monoclonal antibody against hypoglycosylated bone sialoprotein with application as biomarker and therapeutic agent in breast cancer skeletal metastasis. 2018;4: 55-68. DOI: https://doi.org/10.1002/cjp2.88
Chen J, Mcculloch C A. & Sodek J J A O O B. Bone sialoprotein in developing porcine dental tissues: cellular expression and comparison of tissue localization with osteopontin and osteonectin. 1993; 38: 241-249. DOI: https://doi.org/10.1016/0003-9969(93)90034-J
Pinero G J, Farach-carson M C, Devoll R E, Aubin J E, Brunn J C& Butler W T JA O O B. Bone matrix proteins in osteogenesis and remodelling in the neonatal rat mandible as studied by immunolocalization of osteopontin, bone sialoprotein, α2HS-glycoprotein and alkaline phosphatase. 1995; 40: 145-155. DOI: https://doi.org/10.1016/0003-9969(94)00144-Z
Arambawatta A K S, Yamamoto T & Wakita M J A O A A A. Immunohistochemical characterization of noncollagenous matrix molecules on the alveolar bone surface at the initial principal fiber attachment in rat molars. 2005; 187: 77-87. DOI: https://doi.org/10.1016/j.aanat.2004.08.003
Ivanovski S, Li H, Daley T & Bartold P J J O P R. An immunohistochemical study of matrix molecules associated with barrier membrane‐mediated periodontal wound healing. 2000; 35: 115-126 DOI: https://doi.org/10.1034/j.1600-0765.2000.035003115.x
Ishigaki R, Takagi M, Igarashi M & Ito K J T H J. Gene expression and immunohistochemical localization of osteonectin in association with early bone formation in the developing mandible. 2002; 34: 57-66. DOI: https://doi.org/10.1023/A:1021352110531
Tera T D M, Nascimento R D, Prado R F D, Santamaria M P & Jardini M A N J J O A O S. Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats. 2014; 22: 541-553. DOI: https://doi.org/10.1590/1678-775720140190
Chen J, Shapiro H S, Sodek J J J O B & research M. 1992. Developmental expression of bone sialoprotein mRNA in rat mineralized connective tissues. 1992; 7: 987-997. DOI: https://doi.org/10.1002/jbmr.5650070816