Experimental In vitro Study to Assess the Antibacte-rial Activity of Thymus vulgaris Oil on Streptococ-cus Sanguinis
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Abstract
Background: The Streptococcus genus are the predominant bacteria in the mouth and the Streptococcus sanguinis is one of them which performing a primary function for expansion of dental biofilm. Gingival and periodontal disorders are caused by dental biofilm, today, there is a necessity to discover naturally presenting antibacterial compounds from herbs with less side effects as a substitutive to the commonly handled chlorohexidine. Thus, the purpose of this study was to assess the antibacterial activity of thymus vulgaris oil on Streptococcus sanguinis bacteria In vitro. Materials and Methods: Human supragingival plaque samples were taken from 10 subjects, then morphological and microscopical examination, biochemical tests, Optochin test, hemolytic ability test and conventional polymerase chain reaction test was applied to assure coincidence of Streptococcus sanguinis. The sensitivity of bacteria to Thymus vulgaris oil, the minimum concentration that inhibit the growth and killed the test bacteria were identified a partly as comparable to 0.2% chlorhexidine as a positive control and10 % dimethyl sulphoxide as a negative control. Results: Thymus vulgaris oil presented a significant antibacterial property on Streptococcus sanguinis with several grades of inhibition zones. When compared to Chlorohexidine, Thymus vulgaris oil had stronger antibacterial properties. The minimum concentration that inhibited the growth and killed Streptococcus sanguinis was (0.09%). Conclusion: Thymus vulgaris oil displayed higher antibacterial action with each concentration on Streptococcus sanguinis as compared to Chlorohexidine, therefore; it can be used as a natural substitutive oral health care product to Chlorohexidine.
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References
Zhou X, Li Y. Atlas of oral microbiology: From healthy microflora to disease: Springer Nature; 2021.
Bathla S. Periodontics revisited: JP Medical Ltd; 2011.
McGrath C, Zhou N, Wong HM. A systematic review and meta-analysis of dental plaque control among children and ado-lescents with intellectual disabilities. 2019;32(3):522-32.
Takenaka S, Ohsumi T, Noiri Y. Evidence-based strategy for dental biofilms: Current evidence of mouthwashes on dental biofilm and gingivitis. Jpn Dent Sci Rev. 2019;55(1):33-40.
Jafer M, Patil S, Hosmani J, Bhandi SH, Chalisserry EP, Anil S. Chemical Plaque Control Strategies in the Prevention of Bio-film-associated Oral Diseases. J Contemp Dent Pract. 2016;17(4):337-43.
Supranoto S, Slot D, Addy M, Van der Weijden G. The effect of chlorhexidine dentifrice or gel versus chlorhexidine mouthwash on plaque, gingivitis, bleeding and tooth discoloration: a systematic review. Int J Dent Hyg. 2015;13(2):83-92.
James P, Worthington HV, Parnell C, Harding M, Lamont T, Cheung A, et al. Chlorhexidine mouthrinse as an adjunctive treatment for gingival health. Cochrane Database Syst Rev. 2017;3(3):CD008676.
Mehta S, Pesapathy S, Joseph M, Tiwari PK, Chawla S. Comparative evaluation of a herbal mouthwash (Freshol) with chlorhexidine on plaque accumulation, gingival inflammation, and salivary Streptococcus mutans growth. J Int Soc Prev Community Dent. 2013;3(1):25–8.
Kryvtsova M, Salamon I, Koscova J, Bucko D, Spivak M. Antimicrobial, antibiofilm and biochemichal properties of Thymus vulgaris essential oil against clinical isolates of opportunistic infections. Biosyst Divers. 2019;27(3):270-5.
Micucci M, Protti M, Aldini R, Frosini M, Corazza I, Marzetti C, et al. Thymus vulgaris L. essential oil solid formulation: chemical profile and spasmolytic and antimicrobial effects. Biomolecules. 2020;10(6):860.
Denepitiya L, Kleinberg I. A comparison of the microbial compositions of pooled human dental plaque and salivary sedi-ment. Arch Oral Biol. 1982;27(9):739-45.
Hoshino T, Kawaguchi M, Shimizu N, Hoshino N, Ooshima T, Fujiwara T. PCR detection and identification of oral strep-tococci in saliva samples using gtf genes. Diagn Microbiol Infect Dis. 2004;48(3):195-9.
Obaid Z. M. AMA-A. Anti-Bacterial Effects of Commiphora Myrrha and Ziziphus Spina-Christ Leaves Extracts Against Streptococcus Mitis (Primary Colonizer of Dental Plaque) In vitro Study. J Res Med Dent Sci. 2020;8(3):57-64.
England P. Identification of Streptococcus species, Enterococcus species and morphologically similar organisms. UK Stand Microbiol Investig. 2014; 4:1-36.
Smith AC, Hussey MA. Gram stain protocols. American society for microbiology. 2005; 1:14.
Reiner K. Catalase test protocol. American society for microbiology. 2010 Nov 11:1-6.
Buxton R. Blood agar plates and hemolysis protocols. American Society for Microbiology. 2005 Sep 30:1-9.
Cavalieri S, Harbeck R, McCarter Y, Ortez J, Rankin I, Sautter R, et al. Manual of antimicrobial susceptibility testing. Amer-ican Society for Microbiology. PAHO: Washington, DC, USA. 2005.
Razak FA, Othman RY, Abd Rahim ZH. The effect of Piper betle and Psidium guajava extracts on the cell-surface hydro-phobicity of selected early settlers of dental plaque. J Oral Sci. 2006;48(2):71-5.
Abdulbaqi HR, Himratul-Aznita WH, Baharuddin NA. Anti-plaque effect of a synergistic combination of green tea and Salvadora persica L. against primary colonizers of dental plaque. Arch Oral Biol. 2016; 70:117-24.
Fani M, Kohanteb J. In vitro antimicrobial activity of Thymus vulgaris essential oil against major oral pathogens. J evid-based complement altern med. 2017;22(4):660-6.
Thosar N, Basak S, Bahadure RN, Rajurkar M. Antimicrobial efficacy of five essential oils against oral pathogens: An in vitro study. Eur J Dent. 2013;7(S 01): S071-S7.
de Carvalho RJ, de Souza GT, Honório VG, de Sousa JP, da Conceição ML, Maganani M, et al. Comparative inhibitory effects of Thymus vulgaris L. essential oil against Staphylococcus aureus, Listeria monocytogenes and mesophilic starter co-culture in cheese-mimicking models. Food Microbiol. 2015; 52:59-65.
Tuttolomondo T, Dugo G, Ruberto G, Leto C, Napoli EM, Cicero N, et al. Study of quantitative and qualitative variations in essential oils of Sicilian Rosmarinus officinalis L. Nat Prod Res. 2015;29(20):1928-34.
Schött G, Liesegang S, Gaunitz F, Gleß A, Basche S, Hannig C, et al. The chemical composition of the pharmacologically ac-tive Thymus species, its antibacterial activity against Streptococcus mutans and the antiadherent effects of T. vulgaris on the bacterial colonization of the in situ pellicle. Fitoterapia. 2017; 121:118-28.
de Oliveira MA, da C Vegian MR, Brighenti FL, Salvador MJ, Koga-Ito CY. Antibiofilm effects of Thymus vulgaris and Hyptis spicigera essential oils on cariogenic bacteria. Future Microbiol. 2021;16(4):241-55.
Rota MC, Herrera A, Martínez RM, Sotomayor JA, Jordán MJ. Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food control. 2008;19(7):681-7.
Liu T, Kang J, Liu L. Thymol as a critical component of Thymus vulgaris L. essential oil combats Pseudomonas aeruginosa by intercalating DNA and inactivating biofilm. LWT. 2021; 136:110354.
Lambert R, Skandamis PN, Coote PJ, Nychas GJ. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol. 2001;91(3):453-62.
Burt S. Essential oils: their antibacterial properties and potential applications in foods--a review. Int J Food Microbiol. 2004;94(3):223-53.
Rudramurthy GR, Swamy MK, Sinniah UR, Ghasemzadeh A. Nanoparticles: alternatives against drug-resistant pathogenic microbes. Molecules. 2016;21(7):836.
Sienkiewicz M, Łysakowska M, Denys P, Kowalczyk E. The antimicrobial activity of thyme essential oil against multidrug resistant clinical bacterial strains. Microb Drug Resist. 2012;18(2):137-48.
Tardugno R, Pellati F, Iseppi R, Bondi M, Bruzzesi G, Benvenuti S. Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria. Nat Prod Res. 2018;32(5):544-51.
Nikolić M, Glamočlija J, Ferreira IC, Calhelha RC, Fernandes Â, Marković T, et al. Chemical composition, antimicrobial, an-tioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. es-sential oils. Ind Crops Prod. 2014; 52:183-90.
Imelouane B, Amhamdi H, Wathelet J-P, Ankit M, Khedid K, El Bachiri A. Chemical composition and antimicrobial activity of essential oil of thyme (Thymus vulgaris) from Eastern Morocco. Int J Agric Biol. 2009;11(2):205-8.
Rodriguez-Garcia A, Galan-Wong LJ, Arevalo-Niño K. Development and in vitro evaluation of biopolymers as a delivery system against periodontopathogen microorganisms. Acta Odontol Latinoam. 2010;23(2):158-63.
Hernndeza T, Canalesa M, Durana A, Garcaa AM, Avilaa JG, Hernndez-Portillab L, et al. Variation in the hexanic extract composition of Lippia graveolens in an arid zone from Mexico: environmental influence or true chemotypes? Open plant sci j. 2009;3(1): 29-34.
Soković MD, Vukojević J, Marin PD, Brkić DD, Vajs V, Van Griensven LJ. Chemical composition of essential oilsof thymus and mentha speciesand their antifungal activities. Molecules. 2009;14(1):238-49.
Al-Badr RJ, Al-Huwaizi HF. Effect of Tea Tree, Thymus Vulgaris and Nigella Sativa Oils on The Elimination of Enterococcus Faecalis (In Vitro Study). J Bagh Coll Dent. 2017;29(1):55-62.