Salivary protein carbonyl and selected antioxidants in relation to dental caries among pregnant women
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Abstract
Background: Pregnancy is a physiological condition that affects the general and oral health.It is also associated with an increase in oxidative stress, which may presispose to oral diseases including dental caries. Aim of the study: This study aimed to measure salivary protein carbonyl, glutathione peroxidase and selenium levels of women who are pregnant and their association with dental caries in comparison to non-pregnant women, and to find out the mostly affected biomarker of oxidative stress during pregnancy. Subjects, materials and methods: A cross-sectional research was performed for a samples of 30 pregnant and 30 non-pregnant women who were chosen from city of Baghdad's Primary Healthcare Centers. Both groups aged 25-30 years. In unstimulated salivary samples protein carbonyl and glutathione peroxidase were determined colorimetrically using spectrophotometer by utilizing ready-made assay kits. Salivary selenium level was obtained by atomic absorption spectrophotometer. Plaque index had been used to determine the thickness of dental plaque. Caries was recorded using the Decayed, Missing, and Filled (DMF) index. described by WHO in 1997. Data was statistically analyzed using descriptive statistics method and Student's t-test, Wilcoxon sum rank test and Spearman’s correlation in addition to Receiver Operating Characteristics Curve (ROC test) (α=5%). Results: The plaque index and salivary protein carbonyl values were significantly higher among pregnant while salivary selenium and glutathione peroxidase recorded significantly lower levels among pregnant women. Dental caries parameters were higher among pregnant with significant difference for MS fraction only. ROC area for protein carbonyl equal one with highest sensitivity and specificity. Conclusion: Pregnant women recorded higher dental caries severity with higher salivary protein oxidation but lowers salivary antioxidant defense mechanisms. Salivary protein carbonyl is more ideal, valid and mostly affected biomarker in revealing the oxidative stress status during pregnancy
Received date: 10-09-2022
Accepted date: 15-11-2022
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References
Skutnik-Radziszewska, A., Maciejczyk, M., Fejfer, K., et al. Salivary antioxidants and oxidative stress in psoriatic patients: can salivary total oxidant status and oxidative status index be a plaque psoriasis biomarker? Oxid Med Cell Longev. 2020; 2020: PMC6964728. DOI: https://doi.org/10.1155/2020/9086024
Lorenzo-Pouso, A.I., Pérez-Sayáns, M., Bravo, S.B., et al. Protein-based salivary profiles as novel biomarkers for oral diseases. Dis Markers. 2018; 2018: 6141845. DOI: https://doi.org/10.1155/2018/6141845
Żukowski, P., Maciejczyk, M., Waszkiel, D. Sources of free radicals and oxidative stress in the oral cavity. Arch Oral Biol. 2018; 92: 8-17. DOI: https://doi.org/10.1016/j.archoralbio.2018.04.018
Balaji, T.M., Vasanthi, H.R., Rao, S.R. Gingival, plasma and salivary levels of melatonin in periodontally healthy individuals and chronic periodontitis patients: a pilot study. J Clin diagn Res. 2015; 9(3): ZC23. DOI: https://doi.org/10.7860/JCDR/2015/11311.5652
Muthukumar, K., Rajakumar, S., Sarkar, M.N., et al. Glutathione peroxidase3 of Saccharomyces cerevisiae protects phospholipids during cadmium-induced oxidative stress. Antonie Van Leeuwenhoek. 2011; 99: 761-71. DOI: https://doi.org/10.1007/s10482-011-9550-9
Zachara, B. Selenium in pregnant women: Mini review. J Nutr Food Sci. 2016; 6(3):1-7.
Uysal, T., Akkurt, M.D., Amasyali, M., et al. Does a chitosan-containing dentifrice prevent demineralization around orthodontic brackets? Angle Orthod. 2011; 81(2): 31925. DOI: https://doi.org/10.2319/062910-359.1
Ahmad, S., Khan, H., Shahab, U., et al. Protein oxidation: an overview of metabolism of sulphur containing amino acid, cysteine. Front Biosci. 2017; 9(1): 71-87. DOI: https://doi.org/10.2741/s474
Fialova, L., Malbohan, I., Kalousova, M., et al. Oxidative stress and inflammation in pregnancy. Scand J Clin Lab Invest. 2006; 66(2): 1218. DOI: https://doi.org/10.1080/00365510500375230
Ahmadi-Motamayel, F., Goodarzi, M.T., Mahdavinezhad, A., et al. Salivary and serum antioxidant and oxidative stress markers in dental caries. Caries Res. 2018; 52(6): 565-9. DOI: https://doi.org/10.1159/000488213
Baltacioglu, E., Sukuroglu, E. Protein carbonyl levels in serum, saliva and gingival crevicular fluid in patients with chronic and aggressive periodontitis. Saudi Dent J. 2019; 31(1): 23-30. DOI: https://doi.org/10.1016/j.sdentj.2018.09.003
Zamani-Ahari, U., Zamani-Ahari, S., Fardi-Azar, Z., et al. Comparison of total antioxidant capacity of saliva in women with gestational diabetes mellitus and non-diabetic pregnant women. J Clin Exp Dent. 2017; 9(11): e1282. DOI: https://doi.org/10.4317/jced.53845
Awn, B.H., Munther, S., Yas, B.A. Salivary protein carbonyl level in relation to gingival health status among a group of Iraqi pregnant women. Ann Trop Med Public Health; 23(S11): SP231129
. Wagle, M., Basnet, P., Vartun, Å., et al. Oxidative stress levels and oral bacterial milieu in the saliva from pregnant vs. non-pregnant women. BMC Oral Health. 2020; 20(1): 1-8. DOI: https://doi.org/10.1186/s12903-020-01230-3
Gümüş, P., Emingil, G., Öztürk, V-Ö., et al. Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum. BMC Infectious Dis 2015; 15(1): 1-9. DOI: https://doi.org/10.1186/s12879-015-1003-z
Rai, B., Kharb, S., Jain, R, et al. Effect of scaling and root planning on salivary 8-hydroxydeoxyguanosine levels: periodontitis. Internet J Dent Sci. 2006; 5(2):1-4. DOI: https://doi.org/10.5580/d43
Popovici, D., Crauciuc, E., Socolov, R., et al. Early diagnosis and treatment of dental caries in pregnancy. Maedica. 2018; 13(2): 101.
Navazesh, M., Kumar, S.K. Measuring salivary flow: challenges and opportunities. J Am Dent Assoc 2008; 139: 35S-40S. DOI: https://doi.org/10.14219/jada.archive.2008.0353
Mutlak, N.Q., Yas, B.A. Dental caries severity in relation to selected salivary variables among a group of pregnant women in Baghdad city/Iraq. J Bagh Coll Dentistry. 2017; 29(2):115-21. DOI: https://doi.org/10.12816/0038760
Organization WH. Oral health surveys: basic methods: World Health Organization; 2013.
Reznick, A.Z., Packer, L. Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol. 1994; 233: 357-63. DOI: https://doi.org/10.1016/S0076-6879(94)33041-7
Solovyev, M., Kashinskaya, E., Izvekova, G., et al. Feeding habits and ontogenic changes in digestive enzyme patterns in five freshwater teleosts. J Fish Biol. 2014; 85(5): 1395-412. DOI: https://doi.org/10.1111/jfb.12489
Al-Najjar, S.N. Selected salivary antioxidants and lipid peroxidation biomarker in relation to periodontal health condition among a group of pregnant women (A Comparative Study). Maser Thesis College of Dentistry, University of Baghdad. 2018.
Iorgulescu, G. Saliva between normal and pathological. Important factors in determining systemic and oral health. J Med Life. 2009; 2(3): 303.
Hershkovich, O., Shafat, I., Nagler, R.M. Age-related changes in salivary antioxidant profile: possible implications for oral cancer. J Gerontol A Biol Sci Med Sci. 2007; 62(4): 361-6. DOI: https://doi.org/10.1093/gerona/62.4.361
Southward, K. The systemic theory of dental caries. General Dent. 2011; 59(5): 367-73.
Salman, B.N., Darvish, S., Goriuc, A., et al. Salivary oxidative stress markers’ relation to oral diseases in children and adolescents. Antioxidants. 2021;10(10):1540. DOI: https://doi.org/10.3390/antiox10101540
Benson, P.E., Douglas, C.I., Martin, M.V. Fluoridated elastomers: effect on the microbiology of plaque. Am J Orthod Dentofacial Orthop. 2004; 126(3): 325-30. DOI: https://doi.org/10.1016/j.ajodo.2003.07.007