Sleep disorders and dental caries related to salivary alpha-amylase among dental students
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Abstract
College students experience several sleep problems, which may impact academic performance and health including dental health. The purpose of the study was to assess the effect of sleep disorders on dental caries status related to salivary alpha-amylase among dental students. Methods: A cross-sectional comparative study was done among dental students, aged 22 to 23 years attending the College of Dentistry, Al- Kufa University, 270 students have participated in this study. A questionnaire completed by those students was used to collect information about sleep disorders using Pittsburg sleep quality index. According to Manji et al (1989) criteria, teeth affected by dental caries were recorded. Unstimulated salivary samples were chemically analyzed for the detection of alpha-amylase using Enzyme Linked Immuno-Sorbent Assay. Results: It was found that the prevalence of poor sleep quality among dental students was 58.9% while the prevalence of dental caries in students with good sleep quality (75.68%) was higher than that for students with poor sleep quality (71.07%), also founded decay,missing and filling surfaces and decay level three of caries severity were significantly higher for students who had a good sleep quality. The mean value of salivary alpha-amylase was higher for students who had poor sleep quality yet difference was not significant. Conclusion: Caries experience was significantly higher among students with good sleep quality that was not affected by salivary amylase.
Received date: 05-05-2022
Accepted date: 25-07-2022
Published date: 15-06-2024
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References
Kapur VK, Redline S, Nieto F, Young TB, Newman AB, Henderson JA. The relation- ship between chronically disrupted sleep and healthcare use. Sleep 2002; 25(3):289–296.
Strine TW, Chapman DP. Associations of frequent sleep insufficiency with health- related quality of life and health behaviors. Sleep Medicine 2005; 6(1):23–27. DOI: https://doi.org/10.1016/j.sleep.2004.06.003
Yang CM, Wu CH, Hsieh MH, Liu MH, Lu FW. Coping with sleep disturbances among young adults: a survey of first-year college students in Taiwan. Behav Med 2003; 29:133–138. DOI: https://doi.org/10.1080/08964280309596066
Gaultney JF. The prevalence of sleep disorders in college students: impact on academic performance. J American Coll Health 2010; 59(2), 91-97. DOI: https://doi.org/10.1080/07448481.2010.483708
Aravinth H, Dhanraj M, Jain AR. Prevalence of sleep disorder among dental students-A questionnaire study. health 2018;10:17.
Lasisid TJ ,Shittu ST, Meludu CC, Salami AA .Differential effects of total and partial sleep deprivation on salivary factors in Wistar rats. Arch Oral Biol 2017; 73:100-4. DOI: https://doi.org/10.1016/j.archoralbio.2016.09.002
Reinhardt ÉL, Fernandes PACM, Markus RP, Fischer FM. Short sleep duration increases salivary IL-6 production. Chronobiol Int 2016; 33:780-2. DOI: https://doi.org/10.3109/07420528.2016.1167710
Arvidsson L, Birkhed D, Hunsberger M, Lanfer A, Lissner L, Mehlig K, et al. BMI, eating habits and sleep in relation to salivary counts of mutans streptococci in children—the IDEFICS Sweden study. Public Health Nutr 2016; 19:1088-92. DOI: https://doi.org/10.1017/S1368980015002347
Watanabe M, Wang DH, Ijichi A, Shirai C, Zou Y, Kubo M, et al. The influence of lifestyle on the incidence of dental caries among 3-year-old Japanese children. Int J Environ Res Public Health 2014; 11:12611- 22. DOI: https://doi.org/10.3390/ijerph111212611
Mattila M, Rautava P, Paunio P, Ojanlatva A, Hyssälä L, Helenius H, et al. Caries experience and caries increments at 10 years of age. CariesRes 2001; 35:435-41. DOI: https://doi.org/10.1159/000047487
O’Leary, ED., Howard, S., Hughes, BM., James, JE., Salivary alpha-amylase reactivity to laboratory social stress with and without acute sleep restriction. J .Psychophysiol 2015; 29, 55–63. DOI: https://doi.org/10.1027/0269-8803/a000134
Seugnet L, Boero J, Gottschalk L, Duntley SP, Shaw PJ. Identification of a biomarker for sleep drive in flies and humans. Proc. Natl. Acad. Sci 2006; 103:19913–19918. DOI: https://doi.org/10.1073/pnas.0609463104
Yamaguchi M, Sakakima J, Evaluation of driver stress in a motor-vehicle driving simulator using a biochemical marker. J. Int. Med. Res 2007; 35, 91–100. DOI: https://doi.org/10.1177/147323000703500109
Yamaguchi M, Deguchi M, Wakasugi J, Ono S, Takai N, Higashi T et al. Hand-held monitor of sympathetic nervous system using salivary amylase activity and its validation by driver fatigue assessment. Biosens. Bioelectron 2006; 21, 1007–1014. DOI: https://doi.org/10.1016/j.bios.2005.03.014
Muehlhan M, Lueken U, Siegert J, Wittchen HU, Smolka MN, Kirschbaum C. Enhanced sympathetic arousal in response to FMRI scanning correlates with task induced activations and deactivations.. PLoS One 2013; 8, e72576. DOI: https://doi.org/10.1371/journal.pone.0072576
Harris N, Garcia-Godoy F, Nathe CN. Primary preventive dentistry. 7th ed. London: Asimon and Schuster Company 2009; 19.
Chaudhuri B, Rojek J, Vickerman M, Tanzer J, Scannapieco F. Interaction of salivary alpha-Amylase and amylase binding-protein A (AbpA) of S. gordonii with glucosyltransferase of S. gordonii and S. mutans. BMC Microbiology 2007; 7: 60. DOI: https://doi.org/10.1186/1471-2180-7-60
Fábián TK, Hermann P, Beck A, Frejérdy P, Fábián G. Salivary defense proteins: their network and role in innate and acquired oral immunity. Int J Mol Sci 2012; 13:4295-320. DOI: https://doi.org/10.3390/ijms13044295
Buysse .DJ, Reynolds III CF, Monk TH, Berman SR, Kupfer DJ. The Pittsburgh Sleep Quality Index: a new instrument for psychiatric practice and research. Psychiatry Res 1989; 28(2):193–213. DOI: https://doi.org/10.1016/0165-1781(89)90047-4
Manji F, Fejerskov O, Baelum V. Pattern of Dental caries in an adult rural population. Caries Research 1989; 23(1), 55-62. DOI: https://doi.org/10.1159/000261155
Khurshid Z, Zohaib S, Najeeb S, Zafar MS, Slowey PD, Almas K. Human Saliva Collection Devices for Proteomics : An Update . Inter J Molec Sci 2016; 17 (6):846. DOI: https://doi.org/10.3390/ijms17060846
Navazesh M., Kumar SKS. Measuring salivary flow: challenges and opportunities. J Amer Dent Assoc. 2008; 139(2): 35-40. DOI: https://doi.org/10.14219/jada.archive.2008.0353
Machado AV, Castro CO, Botelho Filho CR, Bruzamolin CD, Scariot R., Pizzatto E, Gabardo MC. Anxiety and sleep quality in dental students at a private Brazilian University. Bullet Tokyo Dent Coll 2020; 61(1), 27-36. DOI: https://doi.org/10.2209/tdcpublication.2018-0072
Dorrian J, Tolley C, Lamond N, van den Heuvel C, Pincombe J, Rogers AE, Drew D. Sleep and errors in a group of Australian hospital nurses at work and during the commute. Applied Ergonomics 2008; 39(5), 605–613. DOI: https://doi.org/10.1016/j.apergo.2008.01.012
Andreae C, Strömberg A, Årestedt K. Prevalence and associated factors for decreased appetite among patients with stable heart failure. J Clin Nur 2016; 25(11-12), 1703-1712. DOI: https://doi.org/10.1111/jocn.13220
Muscaritoli M, Anker SD, Argilés J, Aversa Z, Bauer JM, Biolo GI, et al. Consensus definition of sarcopenia, cachexia and pre-cachexia: joint document elaborated by Special Interest Groups (SIG) cachexia-anorexia in chronic wasting diseases and nutrition in geriatrics. Clin nutrition 2010; 29(2), 154-159. DOI: https://doi.org/10.1016/j.clnu.2009.12.004
Redeker NS, Jeon S, Muench U, Campbell D, Walsleben J, Rapoport DM. Insomnia symptoms and daytime function in stable heart failure. Sleep 2010; 33(9), 1210-1216. DOI: https://doi.org/10.1093/sleep/33.9.1210
AM GS, Nieto, G. Dental health: relationship between dental caries and food consumption. Nutricion Hospitalaria 2013; 28, 64-71.
Diajil A, Sood L, Azeez R. A Salivary α-Amylase Level in Relation to the Oral Health Parameters among Children in Baghdad City. J Bagh Coll Dent 2016; 28(2): 40-46 DOI: https://doi.org/10.12816/0028212
Yuen HK, Hant FN, Hatfield C, Summerlin LM, Smith EA, Silver RM. Factors associated with oral hygiene practices among adults with systemic sclerosis. Inter J dent hygiene 2014; 12(3): 180-186. DOI: https://doi.org/10.1111/idh.12056
Chanamanee P, Taboonpong S, Intanon T. Sleep quality and related factors among university students in southern Thailand. Songklanagarind Med J 2020; 24(3): 163-173
Lund HG, Reider BD, Whiting AB, Prichard JR. Sleep patterns and predictors of disturbed sleep in a large population of college students. J adol health 2010; 46(2):124-132. DOI: https://doi.org/10.1016/j.jadohealth.2009.06.016
Bosch JA, Veerman EC, de Geus EJ, Proctor GB. α-Amylase as a reliable and convenient measure of sympathetic activity: don’t start salivating just yet!. Psychoneuroendocrinology 2011; 36(4), 449-453.. DOI: https://doi.org/10.1016/j.psyneuen.2010.12.019
De Farias D, Bezerra AB. Salivary antibodies, amylase and protein from children with early childhood caries. Clin Oral Invest 2003; 7:154–157. DOI: https://doi.org/10.1007/s00784-003-0222-7
Carra MC, Schmitt A, Thomas F, Danchin N, Pannier B, Bouchard P. Sleep disorders and oral health: a cross-sectional study. Clin oral investig 2017; 21(4):975-983 . DOI: https://doi.org/10.1007/s00784-016-1851-y