Salivary Cortisol as a Stress Biomarker and Total Viable Count of Salivary Bacterial Microbiome among COVID-19 Patients
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Abstract
Materials and methods: a sample of 84 adults patients was collected who were divided into two groups: the COVID-19 group consists of 42 patients and the COVID-19 free group which consists of 42 subjects. All subjects undergo a PCR test to confirm their health status. The collection of Un-stimulated saliva was done. Laboratory investigations were carried out to measure the total viable count of the salivary bacterial microbiome by culturing on Brain Heart Infusion Agar and to evaluate the salivary cortisol level using cortisol kit (Elecsys Cortisol II).
Results: SPSS version 21 was used for statistical analysis. According to the statistical analysis, the salivary cortisol and total viable count of salivary bacterial microbiome values were substantially greater in the COVID-19 group than in the COVID-19 free group.
Conclusion: A positive association was found between salivary cortisol and the total viable count of the salivary bacterial microbiome. So, when the concentration of salivary cortisol is elevated in the COVID-19 group, the level of the total viable count of the salivary bacterial microbiome is also elevated.
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References
Palmer-Bouva, C., Oosting, J., deVries, R. et al. Stress in elective dental treatment: epinephrine, norepinephrine, the VAS, and CDAS in four different procedures. Gen Dent. 1998; 46:356–360.
Griffiths, HR., Moller, L. Biomarkers. Mol Aspects Med. 2002; 23, 101–208.
McEwen, BS. Biomarkers for assessing population and individual health and disease related to stress and adaptation. Metabolism. 2015; 64, S2–S10.
Naylor, S. Biomarkers: current perspectives and future prospects. Expert Rev. Mol. Diagn. 2003; 3, 525–529.
Sahu, P., Pinkalwar, N., Dubey, RD., et al. Biomarkers: an emerging tool for diagnosis of a disease and drug development. Asian J. Res. Pharm. Sci.2011; 1, 9–16.
Dhama K, Shyma K., Dadar ML, et al. Biomarkers in Stress Related Diseases/Disorders: Diagnostic, Prognostic, and Therapeutic Values, 2019.
Clow A, Hucklebridge F, Thorn L. The cortisol awakening response in context. Int Rev Neurobiol; 2010; 93:153-75.
Hellhammer, D., Wust, S., Kudielka, M. Salivary cortisol as a biomarker in stress research. Psychoneuroendocrinol. 2009; 34:163-71.
Hasan, R., Ahmed, J. Determination of the effect of stress on the salivary cortisol level among sample of university students having myofacial pain. JBCD,2013.
Groschl, M. Current status of salivary hormone analysis. Clin. Chem. 2008; 54,1759–1769.
Lightman, S., Terry, JR. The importance of dynamic signalling for endocrine regulation and drug development: relevance for glucocorticoid hormones, 2014.
Lee, S., Kim, H., Youm, J., et al. Non-genomic effect of glucocorticoids on cardiovascular system. Arch. Eur. J. Physiol.2012; 464:549–559.
Berger G, Bitterman R, Azzam Z. The human microbiota: the rise of an ―empire‖. Rambam Maimonides Med J.2015; 6: 1–5.
Edlund A, Santiago-Rodriguez TM, Boehm TK, et al. Bacteriophage and their potential roles in the human oral cavity. J Oral Microbiol. 2015; 7: 27423.
Dewhirst FE, Chen T, Izard J, et al. The human oral microbiome. J bacteriol. 2010; 192(19):5002-17.
Zarco, M., Vess, T., Ginsburg, GS. The oral microbiome in health and disease and the potential impact of personalized dental medicine. Oral Dis. 2012; 18:109–20.
Tikhonova S, Booij L, D'Souza V, et al. Investigating the association between stress, saliva and dental caries: a scoping review. BMC Oral Health. 2018 Mar 13;18(1):41.
Boyce, W., Den Besten, P., Stamperdahl, J., et al. Social inequalities in childhood dental caries: the convergent roles of stress, bacteria and disadvantage. Soc Sci Med. 2010; 71(9):1644–52.
Jain, M., Singh, A., Ankur S. Relationship of Perceived Stress and Dental Caries among Pre University Students in Bangalore City. J. Clin. Diagnostic Res. 2014; 10,7860/11664.