In vitro cytotoxic effect of annona squamosa pulp ex-tract as a mouthwash for children on human normal cell line

Main Article Content

Sumaia Hussein Ali
Zainab Juma Jafar

Abstract

Background: Fruits and their by-products are the primary sources of bioactive chemicals in plants. Because of its phytochemical richness, Annona squamosa fruits have gained the alertness of people willing in health-promoting diets. The purpose of this in vitro study was to evaluate the cytocompatibility effect of ethanolic crude extract of Annona squamosa pulp against a human normal cell line as a mouthwash for children. Material and method: The ethanolic extract of Annona squamosa pulp was extracted using the ultrasonic method and then lyophilized to make it powder. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) test was performed to investigate the cytotoxic activity of the pulp extract on a human normal cell line derived from human dermal fibroblast, neonatal (HDFn). Plates were then incubated with 5% CO2 at 37°C For the following concentrations (400, 200, 100, 50, 25, 12.5, 6.25 g/ml). Each concentration, as well as the positive control chlorhexidine, and the negative control cells without tested material, were tested in triplicate. Results: No significant difference was found between the cytotoxicity of the ethanol crude extract of Annona squamosa and a chlorhexidine (P = >0.05) against human dermal fibroblast of neonate cells, with IC50 (50% growth inhibition of cells) values of 235.4 μg/mL while chlorhexidine had an IC50 of 97.8 μg/mL. Conclusion: Annona squamosa extract is more safe and has less cytotoxicity than chlorhexidine. So, to overcome the problems of chlorhexidine, herbal mouthwash formulations could be utilized as an al-ternative mouthwash.

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1.
Ali SH, Jafar ZJ. In vitro cytotoxic effect of annona squamosa pulp ex-tract as a mouthwash for children on human normal cell line. J Bagh Coll Dent [Internet]. 2022 Mar. 15 [cited 2024 Nov. 21];34(1):60-6. Available from: https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/3093
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Research Articles

How to Cite

1.
Ali SH, Jafar ZJ. In vitro cytotoxic effect of annona squamosa pulp ex-tract as a mouthwash for children on human normal cell line. J Bagh Coll Dent [Internet]. 2022 Mar. 15 [cited 2024 Nov. 21];34(1):60-6. Available from: https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/3093

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References

Deshpande, A., Deshpande, N., Raol, R, et al. Effect of green tea, ginger plus green tea, and chlorhexidine mouthwash on plaque-induced gingivitis: A randomized clinical trial. J Indian Soc Periodontol. 2021; 25(4):307. DOI: https://doi.org/10.4103/jisp.jisp_449_20

Vinod, KS., Sunil, KS., Sethi, P., et al. A novel herbal formulation versus chlorhexidine mouthwash in efficacy against oral microflora. J Int Soc Prev Community Dent. 2018; 8(2):184. DOI: https://doi.org/10.4103/jispcd.JISPCD_59_18

Sharma, H., Kumar, A. Ethnobotanical studies on medicinal plants of Rajasthan (India): A review. J Med Plants Res. 2011;5(7):1107-1112.

Wang, DS., Rizwani, GH., Guo, H., et al. Annona squamosa Linn: cytotoxic activity found in leaf extract against human tumor cell lines. Pak J Pharm Sci. 2014; 27(5):1559-1563.

Gurib-Fakim, A. Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med. 2006; 27(1):1-93. DOI: https://doi.org/10.1016/j.mam.2005.07.008

Gajalakshmi, S., Vijayalakshmi, S., Devi, RV. Phytochemical and pharmacological properties of Annona muricata: a review. Int J Pharm Pharm Sci. 2012; 4(2):3-6.

Wunderlin, R., Hansen, B. Synonyms of Annona squamosa. Atlas of Florida Vascular Plants. 2008:24-27.

Das, S., Bhattacharya, A., Ghosh, B., et al. Analytical and Phytochemical Exploration of the Seeds of Annona squamosa. J Anal Pharm Res 2016; 3(4):00065-00070. DOI: https://doi.org/10.15406/japlr.2016.03.00065

Vyas, K., Manda, H., Sharma, RK., et al. An update review on Annona Squamosa. Int J Pharm Therapeut. 2012; 3(2):107-118.

Pandey, N., Barve, D. Phytochemical and pharmacological review on Annona squamosa Linn. Int J Res Pharm Biomed Sci. 2011; 2(4):1404-1412.

Chimbevo, LM. Efficacy and Safety, Nutritional and Antioxidant Activity of Kenyan Annona muricata (L.) and Annona squamosa (L.) Fruits Extracts in BALB/c Mice Model of L. major Leshmaniasis (Doctoral dissertation, JKUAT-COHES), 2020.

Gupta, RK., Kesari, AN., Watal, G., et al. Nutritional and hypoglycemic effect of fruit pulp of Annona squamosa in normal healthy and alloxan-induced diabetic rabbits. Ann Nutr Metab. 2005; 49(6):407-413. DOI: https://doi.org/10.1159/000088987

Tong, SY., Davis, JS., Eichenberger, E., et al. Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015; 28(3):603-661 DOI: https://doi.org/10.1128/CMR.00134-14

Ahmad, H., Ali, N., Ahmad, B., et al. Screening of solanum surrattense for antibacterial, antifungal, phytotoxic and hae-magglutination. J Tradit Chin Med. 2012; 32(4):616-620. DOI: https://doi.org/10.1016/S0254-6272(13)60080-1

Adan, A., Kiraz, Y., Baran, Y. Cell proliferation and cytotoxicity assays. Curr Pharm Biotechnol. 2016; 17(14):1213-1221. DOI: https://doi.org/10.2174/1389201017666160808160513

Decker, T., Lohmann-Matthes, ML. A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods. 1988; 115(1):61-69. DOI: https://doi.org/10.1016/0022-1759(88)90310-9

Fotakis, G., Timbrell, JA. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett. 2006; 160(2):171-177. DOI: https://doi.org/10.1016/j.toxlet.2005.07.001

Rogero, SO., Lugão, AB., Ikeda, TI., et al. Teste in vitro de citotoxicidade: estudo comparativo entre duas metodologias. Mater Res.. 2003; 6(3): 317-320. DOI: https://doi.org/10.1590/S1516-14392003000300003

Trintinaglia, L., Bianchi, E., Silva, LB., et al. Cytotoxicity assays as tools to assess water quality in the Sinos River basin. Braz J Biol. 2015; 75(2):75-80. DOI: https://doi.org/10.1590/1519-6984.0113

Trindade, ML., Radünz, M., Ramos, AH., et al. Chemical characterization, antimicrobial and antioxidant activity of sug-ar-apple (Annona squamosa L.) pulp extract. Rev Chil Nutr. 2020; 47(2):281-285. DOI: https://doi.org/10.4067/S0717-75182020000200281

Freshney, RI. 'Culture of Animal Cells, A Manual Of Basic Technique And Specialized Applications, sixth ed., Wiley- Blackwell, 2010. DOI: https://doi.org/10.1002/9780470649367

Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods. 1983; 65: 55–63. DOI: https://doi.org/10.1016/0022-1759(83)90303-4

Ebisawa, K., Kato, R., Okada, M., et al. Gingival and dermal fibroblasts: their similarities and differences revealed from gene expression. J Biosci Bioeng. 2011; 111(3): 255-258. DOI: https://doi.org/10.1016/j.jbiosc.2010.11.014

Berridge, MV., Herst, PM., Tan, AS. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Bi-otechnol Annu Rev. 2005; 11: 127-152. DOI: https://doi.org/10.1016/S1387-2656(05)11004-7

FDA: Pesticide Analytical Manual Volume I (PAM) 3rd Edition find in https://www.fda.gov/food/laboratory-methods-food/pesticide-analytical-manual-volume-i-pam-3rd-edition.

Karbach, J., Ebenezer, S., Warnke, PH., et al. Antimicrobial effect of Australian antibacterial essential oils as alternative to common antiseptic solutions against clinically relevant oral pathogens. Clin Lab.2015;61(1-2):61-68. DOI: https://doi.org/10.7754/Clin.Lab.2014.140714

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