In vitro cytotoxic effect of annona squamosa pulp ex-tract as a mouthwash for children on human normal cell line

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Published: 2022-03-15
DOI: https://doi.org/10.26477/jbcd.v34i1.3093
Keywords:
Mosmann’s Tetrazolium Toxicity assay (MTT assay), cytotoxicity, Annona squamosa, chlorhexidine, human dermal fibroblast of neonate (HDFn) cell line.

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Sumaia Hussein Ali
Master student, Department of Pediatric and Preventive Dentistry, College of Dentistry, University of Baghdad.
Zainab Juma Jafar
Assistant Professor, Department of Pediatric and Preventive Dentistry, College of Dentistry, University of Baghdad. Bab-Almoadham, P.O. Box 1417, Baghdad, Iraq

Abstract

Background: Fruits and their by-products are the primary sources of bioactive chemicals in plants. Because of its phytochemical richness, Annona squamosa fruits have gained the alertness of people willing in health-promoting diets. The purpose of this in vitro study was to evaluate the cytocompatibility effect of ethanolic crude extract of Annona squamosa pulp against a human normal cell line as a mouthwash for children. Material and method: The ethanolic extract of Annona squamosa pulp was extracted using the ultrasonic method and then lyophilized to make it powder. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) test was performed to investigate the cytotoxic activity of the pulp extract on a human normal cell line derived from human dermal fibroblast, neonatal (HDFn). Plates were then incubated with 5% CO2 at 37°C For the following concentrations (400, 200, 100, 50, 25, 12.5, 6.25 g/ml). Each concentration, as well as the positive control chlorhexidine, and the negative control cells without tested material, were tested in triplicate. Results: No significant difference was found between the cytotoxicity of the ethanol crude extract of Annona squamosa and a chlorhexidine (P = >0.05) against human dermal fibroblast of neonate cells, with IC50 (50% growth inhibition of cells) values of 235.4 μg/mL while chlorhexidine had an IC50 of 97.8 μg/mL. Conclusion: Annona squamosa extract is more safe and has less cytotoxicity than chlorhexidine. So, to overcome the problems of chlorhexidine, herbal mouthwash formulations could be utilized as an al-ternative mouthwash.

Received date: 15-11-2021
Accepted date: 28-12-2021

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Issue

Vol. 34 No. 1 (2022)

Section

Research Articles
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How to Cite

1.
Ali SH, Jafar ZJ. In vitro cytotoxic effect of annona squamosa pulp ex-tract as a mouthwash for children on human normal cell line. J Bagh Coll Dent [Internet]. 2022 Mar. 15 [cited 2025 Dec. 19];34(1):60-6. Available from: https://jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/3093
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